EGCG fatty acid synthesis

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Results 138 of about 39 citing Berger: The new life of a centenarian: signalling functions of NAD (P).. (0.03 seconds) 

Evaluation of Epigallocatechin Gallate and Related Plant Polyphenols as Inhibitors of the FabG and …Find It @ MUgroup of 5 »
YM Zhang, CO Rock – Journal of Biological Chemistry, 2004 –
Institution: Google Indexer Sign In as Member/Non-Member. Originally published In
Press as doi:10.1074/jbc.M403697200 on May 7, 2004 J. Biol. Chem., Vol.
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NAD surfaces againgroup of 4 »
M Ziegler, M Niere – Biochem. J, 2004 –
Related material: PubMed record. PubMed articles by: Ziegler, M. Niere, M.
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NAD+ and its metabolites serve important functions in intracellular signalling. NAD+-mediated regulatory processes also take place on the cell surface, particularly of immune cells. In this issue of the Biochemical Journal, Gerth et al. have demonstrated a new mechanism of Ca2+ uptake into monocytes which is triggered by NAD+ or its degradation product, ADP-ribose. These observations point to a hitherto unknown Ca2+-influx mechanism and underscore the potential significance of NAD+ and ADP-ribose as signalling molecules on the extracellular side of the plasma membrane.

Keywords: ADP-ribose, calcium, ion transport, immune cell, NAD+, pyridine nucleotide

Evaluation of Epigallocatechin Gallate and Related Plant Polyphenols as Inhibitors of the FabG and FabI Reductases of Bacterial Type II Fatty-acid Synthase

Poly (ADP-ribosylation) and genomic stabilitygroup of 3 »
SL Oei, C Keil, M Ziegler – Biochem. Cell Biol, 2005 –
Page 1. 263 REVIEW / SYNTHÈSE Poly(ADP-ribosylation) and genomic stability
1 Shiao Li Oei, Claudia Keil, and Mathias Ziegler Abstract
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GW Templeton, GBG Moorhead – Plant Cell, 2004 –
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Templeton, G. Moorhead, G. Plant Cell.
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A close look at NAD biosynthesisFind It @ MU
N Conferences, D Discovery – Nature Structural & Molecular Biology, 2006 –
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Antioxidant Protective Mechanisms against Reactive Oxygen Species (ROS) Generated by Mitochondrial …group of 3 »
I Hanukoglu – Drug Metabolism Reviews, 2006 – Taylor & Francis
Page 1. Drug Metabolism Reviews, 38: 171–196, 2006 Copyright © Taylor &
Francis Group, LLC ISSN: 0360-2532 print / 1097-9883 online
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Yong-Mei Z hang and Charles O. Rock{ddagger}

From the Protein Science Division, Department of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, Tennessee 38105

Epigallocatechin gallate (EGCG) is the major component of green tea extracts and possesses antibacterial, antiviral, and antitumor activity. Our study focused on validating the inhibition of the bacterial type II fatty acid synthesis system as a mechanism for the antibacterial effects of EGCG and related plant polyphenols. EGCG and the related tea catechins potently inhibited both the FabG and FabI reductase steps in the fatty acid elongation cycle with IC50 values between 5 and 15 µM. The presence of the galloyl moiety was essential for activity, and EGCG was a competitive inhibitor of FabI and a mixed type inhibitor of FabG demonstrating that EGCG interfered with cofactor binding in both enzymes. EGCG inhibited acetate incorporation into fatty acids in vivo, although it was much less potent than thiolactomycin, a validated fatty acid synthesis inhibitor, and overexpression of FabG, FabI, or both did not confer resistance. A panel of other plant polyphenols was screened for FabG/FabI inhibition and antibacterial activity. Most of these inhibited both reductase steps, possessed antibacterial activity, and inhibited cellular fatty acid synthesis. The ability of the plant secondary metabolites to interfere with the activity of multiple NAD(P)-dependent cellular processes must be taken into account when assessing the specificity of their effects.


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